Fig 10. Examples for tethering via dual affinity proteins.

(A) Ptc5 transits mitochondria to peroxisomes and supports tethering. After processing by the peptidase Imp1, Ptc5 is imported into peroxisomes or in the mitochondrial intermembrane space [11]. (B) Lys12 contains a PTS1 that affects proximity between peroxisomes and mitochondria. Translocation to peroxisomes was not observed. (C) Other dual affinity proteins such as Pxp2 contribute to the formation of contacts between peroxisomes and mitochondria and are dually targeted. If transit from one to the other organelle occurs is unknown. (D) Pex15 can directly target peroxisomes or transit through the ER. ER localization promotes tethering. ER, endoplasmic reticulum; PTS, peroxisome targeting signal.