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. 2024 Mar 1;10(9):eadj4698. doi: 10.1126/sciadv.adj4698

Table 1. List of commercial FACS antibodies/reagents used for cell staining.

Reagent Vendor Catalog
Cell Staining Buffer BioLegend 420201
Zombie Aqua Fixable Viability Kit BioLegend 423102
Zombie NIR Fixable Viability Kit BioLegend 423106
CellTrace Violet Cell Proliferation Kit Invitrogen C34557
eBioscience CFSE Invitrogen 65-0850-84
Purified hCD3 antibody (OKT3) BioLegend 317302
PE-hCD3 antibody BioLegend 300308
PB-hCD3 antibody BioLegend 300330
PerCP/Cy5.5-human CD3 antibody BioLegend 344808
APC/Fire 750-human CD4 antibody BioLegend 300560
BV711-human CD8 antibody BioLegend 344734
PE-Cy7 human CD19 antibody BioLegend 302216
APC-Fire750 hCD14 antibody BioLegend 367120
BV711-hCD16 antibody BioLegend 302044
Human UNC5A antibody Proteintech 20239-1-AP
Human UNC5B antibody Abcam ab104871
Human UNC5C antibody Proteintech 20240-1-AP
Human UNC5D antibody Proteintech 20241-1-AP
Human LPHN1 pAb Invitrogen PA5-77475
Human LPHN3 pAb Proteintech 20045-1-AP
APC Human PD-1 BioLegend 621610
PE-Cy7 Human Tim-3 BioLegend 119716
BV421 Human Lag-3 BioLegend 369314
Human TruStain FcR blocker BioLegend 422302
Mouse TruStain FcR blocker BioLegend 101320
PB-human CD45 antibody BioLegend 368540
PE-Cy7 mouse CD45 antibody BioLegend 147703
AF647–anti-mouse IgG Fc Jackson ImmunoResearch 115-606-071
AF647–anti-human IgG (H+L) Thermo Fisher Scientific A21445
PE–anti-rabbit IgG Ab BioLegend 406421
Rabbit IgG isotype (purified) BioLegend 910801
Mouse IgG1 isotype (purified) BioLegend 401402
AF647–anti-rabbit IgG (H+L) Thermo Fisher Scientific A21244
AF647–anti-mouse IgG (H+L) Thermo Fisher Scientific A21235
PE streptavidin BioLegend 405204
Commercial human FLRT3 antibody R&D Systems AF2795
Anti-goat IgG (H+L) PE-conjugated antibody R&D Systems F0107