FIG. 2.
(A to C) Wild-type ovaries hybridized with a digoxigenin-labeled probe to visualize Bic-C RNA. Note the accumulation of Bic-C RNA in a single cell from the earliest vitellarial stages of oogenesis. (D to F) Wild-type ovaries stained with anti-BIC-C antibody. For immunohistochemistry, an antibody raised against a His-tagged 281-amino-acid portion of BIC-C was used (see Materials and Methods for details). (D) Stages of oogenesis from germarium to stage 8; stages are those described by King (24). Oocyte accumulation of BIC-C first becomes detectable at stage 4. (E) Stage 10 egg chamber showing abundant BIC-C protein in the nurse cells. (F) Stage 12 egg chamber showing abundant BIC-C protein in the nurse cells. (G and H) Bic-CAA4 ovaries stained with the same antiserum as in panels D to F. Note the absence of localized signal.