FIG. 1.

Characterization of the anti-Qk1 antibody. (A) Total cell lysates from HeLa cells transfected with vector (none), myc-Qk1 (Qk1), or myc-Sam68 (Sam68) were separated by SDS-PAGE. The proteins were transferred to nitrocellulose and immunoblotted with anti-myc, normal rabbit serum (NRS), anti-Qk1, or anti-Qk1 antibodies preabsorbed with 1 μg of GST-Qk1KH antigen/ml (anti-Qk1/Qk1 KH). The positions of Qk1 and Sam68 are shown on the left, and those of molecular mass markers (in kilodaltons) are on the right. (B) Total cell extracts from rat C6 glioma cells and rat astrocytes were immunoblotted with anti-Qk1. The presence of three Qk1-immunoreactive proteins with approximate molecular masses of 45, 40, and 38 kDa is shown.