FIG. 5.

Association of Qk1 in vitro and localization of the minimal region to amino acids 18 to 57. (A) HA-Qk1 or HA-Qk1:E➛G was transfected into HeLa cells. The cells were lysed, and GST pull-down assays were performed with full-length Qk1 or Qk1:E➛G expressed as a GST fusion protein. The bound proteins were separated by SDS-PAGE, transferred to nitrocellulose, and immunoblotted with anti-HA antibodies. (B) HA-Qk1 was expressed in HeLa cells and incubated with various Qk1 GST fusion proteins as indicated. The bound HA-Qk1 was analyzed as described for panel A. The migration of HA-Qk1 or HA-Qk1:E➛G is shown on the left. TCL, total cell lysate.