TABLE 3.
Hemopoietic parameters of wild-type and Cbl−/− mice
| Parameter | Value (± SD) for genotype:
|
P value | |
|---|---|---|---|
| +/+ | −/− | ||
| Blood | |||
| Leukocytes (106/ml) | 8.5 ± 1.2 | 10.9 ± 1.3 | NSb |
| Erythrocytes (109/ml) | 9.3 ± 0.5 | 9.3 ± 0.6 | NS |
| Platelets (109/ml) | 1,091 ± 39 | 1,755 ± 87 | <0.001 |
| Reticulocytes (109/ml) | 0.39 ± 0.03 | 0.40 ± 0.06 | NS |
| Packed cell vol (%) | 46.9 ± 1.2 | 46.3 ± 0.9 | NS |
| Spleen | |||
| Wt (mg) | 79.6 ± 5.8 | 136.6 ± 9.7 | <0.001 |
| CFC/104 cells | 30.5 ± 8.6 | 65.8 ± 17.8 | NSc |
| Bone marrow | |||
| Cells/femur (107) | 2.3 ± 0.3 | 2.0 ± 0.2 | NS |
| CFC/femur (106)a | 5.55 ± 1.54 | 6.02 ± 1.38 | NS |
| Thymus cellularity (107) | |||
| 5–5.5 wk old | 16.3 ± 2.0 | 24.8 ± 1.3 | <0.005 |
| 7–9 wk old | 16.1 ± 1.6 | 14.9 ± 1.5 | NS |
| Lymph node cellularity (106) | 16.3 ± 2.2 | 31.7 ± 3.7 | 0.004 |
a
CFC responsive to CSF-1 alone were assayed as an index of the committed progenitor cell compartment (5).
b
NS, not significant.
c
The incidence of spleen CFC appeared elevated in Cbl−/− mice but did not reach statistical significance (P = 0.1).