Fig. 2.

Peri-LC NPS⁺ neurons have minimal role in regulating sleep. (A) Schematic of TeNT inhibition of peri-LC NPS⁺ neurons. (B–D) Total wakefulness time (B), NREM sleep time (C), and REM sleep time (D) by EEG/EMG within 24 h, light phase and dark phase were calculated in YFP (n = 6) and TeNT (n = 6) injected mice. (E–G) Total wakefulness (E), NREM sleep (F), and REM sleep (G) were plotted hourly for 24 h in YFP (n = 6) and TeNT (n = 6) injected mice. (H) Schematic slice of chemogenetic activation of peri-LC NPS⁺ neurons. (I–K) Quantified results for total wakefulness (I), NREM (J), and REM (K) sleep recorded 4 h after saline or CNO injection at ZT6 in the hM3Dq (n = 5) injected mice. n.s. = not significant. Two-tailed Student’s t test for (B–D). Two-way ANOVA, post hoc Sidak’s multiple comparisons test for (E–G). RM one-way ANOVA, post hoc Dunnett's multiple comparisons test for (I–K). Error bars represent ± SEM. (Scale bar, 200 μm.)