Fig. 3.

DGR reverse transcriptases (RT) from Thiohalocapsa PB-PSB1 are monophyletic with RTs from other multicellular bacteria and syntenic with conflict system domains associated with programmed cell death. The maximum likelihood phylogeny includes all clade 5 RT proteins from ref. 21, excluding metagenomic and SAG sequences not found in the IMG database [IQ-Tree v1.5.5 (38) built-in model selection: Q.pfam+F+R10; 1,000 bootstraps]. The three layers of annotations indicate (from the inside to the outside): 1) the organism taxonomy, 2) the type of cellular arrangement, and 3) the domains of interest detected within 20 kb of the RT. Domains abbreviations and hits in each organism are provided in Dataset S6. The branches shaded in pink correspond to PB-PSB1’s DGR RTs. Bootstrap support greater than 70% is indicated with closed circles (n = 1,000). Scale bar: 1 substitution per site.