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. 2024 Feb 14;25(3):512–524. doi: 10.1038/s41590-024-01755-7

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 2 — (a) Gating strategy to FACS sort γδ T cells and Treg cells from LNs of Foxp3^Cre-YFPIl23r^fl/fl and Il23r^fl/fl mice for qPCR and example plot showing purity of sorted Treg cells. (b) Bar graphs depicting relative mRNA expression level of Il23r normalized to pol.2 in FACS sorted γδ T cells and Treg cells from steady state LNs of Foxp3^Cre-YFPIl23r^fl/fl, Il23r^fl/fl and Il23r^del/del mice. Data shown from one representative experiment out of two independent experiments with n = 5 (Foxp3^Cre-YFPIl23r^fl/fl and IL23R^fl/fl mice) or n = 3 (Il23r^del/del) biologically independent samples. Data are displayed as mean +/- SEM. Statistical significance was determined using t-tests. (c) Il23r^fl/fl and Foxp3^Cre-YFPIl23r^fl/fl mice were inoculated i.d. with B16 tumor cells, inoculated s.c. with YUMMER1.7 tumor cells or inoculated s.c. with MC38 tumor cells. Tumors were analyzed on day 15, 14 or 24 post-inoculation. Pictures depict tumors after harvest. Data from 3 independent experiments with n = 6-10. (d) Kinetics of tumor volume measured by caliper gauge in B16 tumor cell-inoculated i.d. Foxp3^Cre-YFP and Foxp3^Cre-YFPIl23r^fl/fl mice. Mean +/- SEM is displayed. Statistical significance was determined using 2-way Anovas. n = 4 biologically independent animals. (e) Kinetics of tumor volume measured by caliper gauge in MC38 tumor cell-inoculated s.c. C57Bl/6 mice i.p. injected either with anti-p19 blocking or isotype antibodies. n = 6 (isotype) and n = 7 (anti p19 antibody) biologically independent animals. Mean +/− SEM is displayed. Statistical significance was determined using 2-way Anovas.

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