Fig. 5. PG16-Db and 3BNC117-Db eliminate cells harboring intact and inducible proviruses.

a, Frequency of intact provirus⁺CD4⁺ T cells after treatment with PMA + I and coculture with autologous NK cells in the presence of H2-Db, PG16-Db, 3BNC117-Db or PG16-Db + 3BNC117-Db. Data are reported as intact HIV-1 copies per million viable CD4⁺ T cells (mean, s.d.) from eight replicates. The significance was determined by one-way ANOVA followed by Dunnett’s test for multiple comparisons: ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, ^****P < 0.0001. b, Average frequencies of intact provirus⁺ CD4⁺ T cells (top left), 5′-deleted provirus⁺ CD4⁺ T cells (bottom far left) and 3′-deleted/hypermutated provirus⁺CD4⁺ T cells (bottom middle left) after treatment with PMA + I and coculture with autologous NK cells as in a (mean, s.d.) across all 11 participants assessed. The significance was determined by one-way ANOVA followed by Dunnett’s test for multiple comparisons: ^*P < 0.05, ^**P < 0.01. Average IUPM viable CD4⁺ T cells (bottom middle right) after treatment with PMA + I and coculture with autologous NK cells as in a (mean, s.d.) across six participants assessed. The significance was determined by paired, two-sided Student’s t-test: ^**P < 0.01. Average frequency of intact provirus⁺ CD4⁺ T cells (top middle) or average IUPM viable CD4⁺ T cells (bottom far right) relative to H2-Db. Data reported as a percentage reduction (mean, s.d.) from eleven or six participants, respectively. The significance was determined by Student’s t-tests against a theoretical mean of 0% reductions: ^***P < 0.001, ^****P < 0.0001.