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. 2024 Jan 26;25(3):462–470. doi: 10.1038/s41590-023-01741-5

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 1 — a, Binding of H2-Db, PG16-Db, 3BNC117-Db to immobilized CD16a recombinant proteins. Both CD16 variants are identical except for a single amino acid substitution at position 158 (F/V). Data reported as the optical density at 450nm (OD450) (mean, SD) from three replicates and were fitted to a dose-response nonlinear regression model. b, Calculated EC₅₀ values for the fitted nonlinear regression model from a reported in ng/mL. c, Binding of H2-Db, PG16-Db, and 3BNC117-Db to peripheral blood lymphocytes, CD3⁻CD56⁺ NK cells, CD3⁺ T cells, and CD19⁺ B cells with or without blocking with a CD16-specific antibody (3G8). PBMCs were stained with 1000 ng/mL of H2-Db, PG16-Db, or 3BNC117-Db. Data reported as the percentage of cells that stained positive for the indicated scDb (mean, SD) from three replicates. d, Expression of CD16 on peripheral blood lymphocytes, CD3^-CD56⁺ NK cells, CD3⁺ T cells, and CD19⁺ B cells. Data reported as the percentage of CD16⁺ cells (mean, SD) from three replicates.