TABLE 1.
Number of cells expressing GFP after cotransfection with various constructs
| Construct | Tetracycline addition | Expt 1
|
Expt
2
|
Expt 3
|
Average gain or loss | |||
|---|---|---|---|---|---|---|---|---|
| No. of GFP-positive cellsa | % Gain or lossb | No. of GFP-positive cells | % Gain or loss | No. of GFP-positive cells | % Gain or loss | |||
| Vector | + | 1,893 | 2,127 | 1,974 | ||||
| − | 1,942 | +2.6 | 2,237 | +5.2 | 2,123 | +7.5 | +5.1 ± 2.0 | |
| Mcl-1(S) | + | 2,925 | 2,941 | 3,690 | ||||
| − | 3,183 | +8.8 | 3,239 | +10.0 | 4,128 | +11.9 | +10.2 ± 1.3 | |
| Mcl-1(AS) | + | 1,633 | 1,770 | 1,917 | ||||
| − | 1,315 | −19.5 | 1,338 | −24.4 | 1,573 | −17.9 | −20.6 ± 2.8 | |
a
Number of cells expressing GFP detected by flow cytometry from 105 transfected cells.
b
[100 × (Number of GFP-positive cells in medium without tetracycline) − (number of GFP-positive cells in medium with tetracycline)]/(number of GFP-positive cells in medium with tetracycline).