TABLE 2.
Percentage of apoptotic cells after cotransfection with CD16/CD7 and various constructs
| Construct | Tetracycline addition | Expt
1
|
Expt 2
|
Expt 3
|
Average % differencec | |||
|---|---|---|---|---|---|---|---|---|
| % of double-positive cellsa | % Differenceb | % of double-positive cells | % Difference | % of double-positive cells | % Difference | |||
| Vector | + | 13.5 | 12.6 | 12.6 | ||||
| − | 10.9 | −2.6 | 12.7 | +0.1 | 14.1 | +1.5 | −0.3 ± 1.7 | |
| Mcl-1(S) | + | 6.6 | 13.0 | 11.0 | ||||
| − | 7.9 | +1.3 | 11.8 | −1.2 | 10.5 | −0.5 | −0.1 ± 1.1 | |
| Mcl-1(AS) | + | 11.0 | 16.9 | 16.2 | ||||
| − | 21.7 | +10.7 | 36.8 | +19.9 | 21.0 | +5.2 | +11.9 ± 6.1 | |
a
After transfection, cells were stained with annexin-V and antibody to CD16 and analyzed by flow cytometry. Between 500 and 1,000 of CD16-positive cells from each condition were analyzed for their reactivity to annexin-V.
b
The percentage of double-positive cells cultured without tetracycline minus the percentage of double-positive cells cultured with tetracycline.
c
Average percent difference from the three independent experiments.