Extended Data Fig. 7. CD8+ T cell depletion does not impair vaccine-induced neutralizing antibody responses.
Cohorts of 7-week-old female K18-hACE2 mice were immunized via IN route with a single dose (2 × 109 vp) of ChAd-Control or ChAd-SARS-CoV-2-S vaccine and treated with anti-CD8 or isotype control antibody via IP and IN route as shown in Fig. 7f. Sera were collected four weeks after immunization, and neutralizing antibody responses were determined against SARS-CoV-2 WA1/2020 D614G (n = 5 for ChAd-Control; n = 10 for ChAd-SARS-CoV-2-S + isotype control antibody and ChAd-SARS-CoV-2-S + anti-CD8 antibody, two experiments, boxes illustrate geometric mean values, dotted lines show the LOD. Statistical analysis: one-way ANOVA with Tukey’s post-test: *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.