Extended Data Fig. 8. Lung pathology in ChAd vaccine-immunized K18-hACE2 mice after XBB.1.5 challenge and depletion of CD8+ T cells.
K18-hACE2 mice were immunized, treated with anti-CD8 depletion or isotype control antibodies, and then challenged with XBB.1.5 as described in Fig. 8a. Hematoxylin and eosin staining of lung sections (a) and corresponding pathological scores using two different analysis modes (see Methods) are shown (b). Images show low- (scale bars, 2.5 mm), medium- (scale bars, 500 µm) and high-power (scale bars, 100 µm) magnification. Right panels at high magnifications (400x) show infected lungs with immune cell infiltrates (arrows), thickened septa and consolidated airspace (asterisks), and foci of immune cells around perivasular and peribronchial spaces (arrow heads). Representative images from n = 2 mice per group. Note, the sections from naïve animals are provided as a comparative reference and are from historical specimens shown in Fig. 5.