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. 2024 Feb 5;56(2):408–421. doi: 10.1038/s12276-024-01168-4

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a MRI images (T2WI) of rat caudal vertebrae after 4 weeks of modeling. b The degenerative grades of IVDs were evaluated according to the Pfirrmann grade based on T2WI images. c Quantitative comparison of the water content of IVD tissues in the different groups. d HE and SO staining of the operated segments. e The degenerative grades based on the histological images. f Representative immunohistochemical images and quantitative analysis of CD68, COX2 and CD206 in each group of rat IVD tissues. Experiments were performed 5 times independently, and the data are presented as the means ± SDs. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns, not significant, with ANOVA. Bin, bindarit.