Fig. 6. Sequence specific dissolution and regrowth of distinct condensates.

A, B Orthogonal DNA strands can be annealed to form orthogonal DNA nanostars that do not interact. B Nanostar 1, labeled with Cy3, is represented in orange and nanostar 2, labeled with FAM, is represented in green. C, D Invaders specific to either Nanostar 1 or Nanostar 2 selectively inhibit their capacity to phase separate while leaving the other unaffected, as shown by microscopy images. E, F Respective Anti-invaders for each design reactivate their capacity to phase separate again. Microscopy images were taken 5 min after the addition of the respective invader or anti-invader strands. Representative images of two experimental replicates. Invader and AI = 1×. Scale bars are 10 μm.