Fig. 1. Diagram of the experimental setup for the air-liquid interface PK/PD biofilm model.

In this diagram, media flows left to right with the aid of peristaltic pumps. The antibiotics are infused into an Erlenmeyer flask that has a fixed volume of growth media by an automated dosing pump (red) housed in a refrigerator (not shown). This flask is located on a magnetic stir plate to mix the antibiotics prior to being pumped into the incubator, where they continuously drip onto the biofilm. The biofilm is grown in a drip flow reactor, which has been previously described to grow the biofilm at the air-liquid interface. Waste media is then removed from the incubator and collected in a reservoir.