Skip to main content
. 1998 Aug;18(8):4899–4913. doi: 10.1128/mcb.18.8.4899

FIG. 4.

FIG. 4

Effects of wild-type or mutant IE1 expression on distribution of the endogenous PML proteins in transient expression assays. Vero cells were transfected with plasmids encoding various mutant IE1 proteins and fixed with paraformaldehyde followed by double-label IFA at 48 h after transfection. (a and b) Paired photographs of cells receiving plasmid pMP17 encoding wild-type IE1(1-491); (c and d) pRL55 encoding both IE1(1-346) and wild-type IE2; (e and f) pRL74 encoding IE1(1-346); (g and h) pRL60 encoding IE1(Δ132-274); (i and j) pRL61 encoding IE1(Δ291-320). (a, c, e, g, and i) Detection of IE1 with mouse MAb 6E1 (a, c, e, and i) or MAb CH810 (g) and FITC-labeled anti-mouse IgG. (b, d, f, h, and j) Detection of PML in the same fields with rabbit anti-PML-C PAb and rhodamine-coupled anti-rabbit IgG.