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. 2024 Mar 1;81(1):110. doi: 10.1007/s00018-024-05155-w

Fig. 5.

Fig. 5

S100A8/9 augments the TLR7-mediated activation of BMDMs by promoting the secretion of IFN-γ. (A-C) BMDMs were treated with mouse recombinant S100A8/9 protein (1 μg/mL) or vehicle for 6 h, and then RNA-seq analysis was performed. A Differential expression genes at FDR < 0.05 in S100A8/9-treated BMDMs versus vehicle-treated BMDMs. B KEGG signaling pathway enrichment analysis of differential gene expression in S100A8/9-treated BMDMs versus vehicle-treated BMDMs. C The changes of the expression of IFN-γ and IFN-inducible genes in S100A8/9-treated BMDMs versus vehicle-treated BMDMs. D, E BMDMs were stimulated with mouse recombinant S100A8/9 protein (1 μg/mL and 5 μg/mL) for 6 or 12 h. The mRNA levels of IFN-γ and IFN-inducible genes were detected by Q-PCR (D). The protein level of IFN-γ was detected by FACS (E). F–H BMDMs were pretreated with mouse recombinant IFN-γ (20 ng/mL) for 12 h, followed by stimulation of R848 for 24 h. Flow cytometric analysis of the expression of CD86 and CD40 (F, G). ELISA analysis of the levels of IL-12/IL-23 p40 and TNF-α in culture supernatant (H). *p < 0.05, **p < 0.01, ***p < 0.001