FIG. 7.

The RFC5 gene can suppress thermosensitive growth caused by the rfc2-1 mutation. (A) The insert DNA fragment carrying a suppressor which was originally isolated and its deletion derivatives are represented by open rectangles. The representative restriction enzyme sites and the open reading frames predicted from the nucleotide sequence of the region are also indicated. The growth phenotype of strain YVN4 (rfc2-1) transformed with different plasmids is shown at the right. (B) Dependence of efficiency of suppression on a copy number of the RFC5 gene. Strains YVN12 (wild type) and YVN13 (rfc2-1) and two transformants of YVN13 harboring either plasmid YCpRFC5 or plasmid YEpRFC5 were streaked onto YPD plates and incubated at different temperatures for 4 days.