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. 2024 Mar 1;14:5135. doi: 10.1038/s41598-024-55143-0

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Correlation between expression of GPRC5D and efficacy of BsAb5003 in several MM cell lines. (a) The expression level of GPRC5D on eight MM cell lines (MM1R, NCI-H929, KMS-26, KMS-28BM, KMS-21 BM, LP-1, U266B1, and KMS-11) was measured by flow cytometry. GPRC5D antibody binding sites/cells were determined using QIFIKIT. Samples were prepared in triplicate and the data represent the mean ± standard deviation. Dotted line represents the lower limit of detection of antibody binding capacity (1500 sites/cell) and U266B1 had a value of 1774 sites/cell, which was just above the detection limit. (b) Correlation between GPRC5D expression level detected by FCM and relative mRNA expression from ddPCR or IHC score. Seven MM cell lines expressing GPRC5D above detectable limit (1500 sites/cell) are plotted. (c) Several MM cell lines were cocultured with hPBMCs at an ET ratio of 5:1 for 3 days, after which target cytotoxicity, CD4 + T cell activation, and CD8 + T cell activation were measured by flow cytometry. The assay was conducted in triplicate and the data represent the mean ± standard error. (d) Correlation diagram between target expression and estimated maximum efficacy of BsAb5003-induced cytotoxicity.