FIG. 4.
Expression pattern of the human H430 retropseudogene. (A) Correspondence of the DNA and RNA probes with the human H430 sequences. (B) An RNase protection analysis of H430 transcripts was performed with the ES165 riboprobe and 7 μg of the indicated polyadenylated RNA. The sizes of the specifically protected fragments are in nucleotides. (C and D) Northern blot analyses of H430 mRNA species. Polyadenylated RNA samples from the indicated human cells lines (5 μg per lane) and normal tissues (2 μg per lane) were hybridized with the 32P-labeled ES165 DNA probe. Transcripts detected in the different samples are indicated by arrows. Sizes are in kilobases. Human GAPDH and β-actin were used as the internal controls to normalize for variations in RNA amounts.