FIG. 5.

Identification of the H430 gene product expressed in vivo. Immunoblot analyses of various extracts or fractions performed with the H430 antibody directed against the P214 peptide (A), the SC35 antibody (B), and MAb 104 (C). Nuclear extracts of HeLa cells (lanes 2) and derived fractions including an ammonium sulfate pellet (60-90P), an Mg²⁺ pellet (Mg²⁺P), and supernatant (Mg²⁺S) were analyzed in parallel with S100 cytoplasmic extract of HeLa cells (S100), nuclear extract of NIH/3T3 cells (NE), and baculovirus-expressed SRp46 (bac. SRp46). The apparent molecular masses of markers are indicated. Note that no signal was obtained in panel A when the H430 antibody was preincubated with an excess of P214 peptide coupled to ovalbumin.