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. 2024 Feb 8;43(5):836–867. doi: 10.1038/s44318-024-00034-3

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the data associated with this article, unless otherwise stated in a credit line to the data, but does not extend to the graphical or creative elements of illustrations, charts, or figures. This waiver removes legal barriers to the re-use and mining of research data. According to standard scholarly practice, it is recommended to provide appropriate citation and attribution whenever technically possible.

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Figure EV3 — The hop1-loop2 allele is simplified to loop2 in figure labels. (A) Sporulation efficiency data for the indicated genotypes. This data represents at least four biological replicates. (B) Number of viable spores per tetrad for each genotype. At least 50 tetrads were dissected from each genotype. (C) Flow cytometry analysis of DNA content, monitoring DNA synthesis to indicate synchronous meiotic entry. This was performed every time samples were prepared from a meiotic yeast culture. (D) Western blots showing protein levels across genotypes for Hop1 protein (Top) (Subramanian et al, 2016), and for phosphorylated Hop1 T318 (Subramanian et al, 2016). This result was observed with at least three biological replicates. Source data are available online for this figure.