Figure 2. Hop1 association to DNA is altered in the absence of nucleosome binding.

The hop1-loop2 allele is simplified to loop2 in figure labels. (A) Spore viability for each genotype. P values represent the Bonferroni-corrected results of unpaired t test between groups indicated by connected lines (N > 100 tetrads per genotype). (B) SNP-ChIP normalized average read counts displayed as a percentage of wild-type. Triangles represent values for each replicate (N = 2). (C) Mean Hop1 ChIP-seq signal per kb is plotted for each chromosome on a log scale with regression analysis and associated Pearson correlation coefficient for each genotype. (D) Hop1 ChIP-seq signal, normalized by SNP-ChIP is plotted along chromosome X for each genotype. Island regions are underlined with black bars and centromere regions are shown as solid triangles. Dotted lines indicate the region depicted in the zoom panel above each chromosome X plot (N = 3). (E) The SNP-ChIP normalized ChIP-sequencing for each genotype is subtracted from wild-type ChIP-seq and then the difference is plotted along chromosome X. All regions above zero indicate regions that contain more Hop1 binding in wild type compared to the corresponding genotype. Island regions are underlined with black bars and centromere regions are shown as open triangles. Source data are available online for this figure.