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. 2024 Feb 8;43(5):836–867. doi: 10.1038/s44318-024-00034-3

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the data associated with this article, unless otherwise stated in a credit line to the data, but does not extend to the graphical or creative elements of illustrations, charts, or figures. This waiver removes legal barriers to the re-use and mining of research data. According to standard scholarly practice, it is recommended to provide appropriate citation and attribution whenever technically possible.

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The hop1-loop2 allele is simplified to loop2 in figure labels. (A) Representative images from chromosome spreads prepared at 3 h after meiotic induction, hybridized with antibodies against Zip1 and Hop1, and stained with DAPI. Scale bars: 1 µm. (B) Dot plot of the quantification of total Hop1 relative fluorescent units (RFU) per cell. Hours 2, 3, and 4 are separated by color as depicted in the legend below. Box plots are overlaid on dot plots and depict interquartile ranges between the 25th and 75th quartile, where the line in the middle of the box represents the medium value. Lines between categories represent P value results; n.s. is not significant. Statistics are determined by unpaired Wilcoxon test with Bonferroni correction, N = 2. (C) Dot plot of the % total DAPI area that the Hop1 signal occupies for each cell. Plot layout, N number, and statistics are the same as (B). Individual quantifications of Hop1 and DAPI area for each cell appear in Appendix Fig. S8. (D) Dot plot of the quantification of total Zip1 RFU per cell. Plot layout, N number, and statistics are the same as (B). (E) Zip1 length for each cell is displayed in micrometers. Plot layout, N number, and statistics are the same as (B). Source data are available online for this figure.