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. 2024 Feb 8;43(5):836–867. doi: 10.1038/s44318-024-00034-3

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the data associated with this article, unless otherwise stated in a credit line to the data, but does not extend to the graphical or creative elements of illustrations, charts, or figures. This waiver removes legal barriers to the re-use and mining of research data. According to standard scholarly practice, it is recommended to provide appropriate citation and attribution whenever technically possible.

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The hop1-loop2 allele is simplified to loop2 in figure labels. (A) Pulsed-field gel and Southern blot analysis of chromosome VIII (analyzed using a probe against CBP2). (B) Percent total broken DNA calculated based on a Poisson distribution using the intact chromosome VIII bands for each genotype and assuming full replication by 6 h (Thacker et al, 2014). Data represents four biological replicates, error bars indicate standard deviation. (C) Southern blot analysis of the GAT1 locus across genotypes during meiosis. The GAT1 hotspot is located outside an island on chromosome 6 (the second smallest chromosome). Top band represents the intact GAT1 locus. Bottom band represents DSBs that occur at the GAT1 locus. (D) Measurements of the DSB induction for each genotype at the GAT1 locus. Data represents two biological replicates for each genotype, with the exception of zip1Δ hop1-loop2 and zip1Δ hop1-loop2 pch2Δ, which were analyzed once. Source data are available online for this figure.