FIG. 5.

HSP90 Abs delay the deactivation of HSF1 in vivo. Uninjected or HSP90 MAb-injected oocytes were subjected to a heat shock at 33°C for 1 h (HS) and then allowed to recover at 18°C for the periods of time indicated, and the HSE-binding activity of HSF1 was analyzed by gel mobility shift assay. Both heat-induced and supershifted complexes are indicated as HSF1 at the left. ns, nonspecific binding activity.