FIG. 7.

(GA) enhances the heat-inducible HSE-binding activity of HSF1 and delays deactivation during recovery. (A) Oocytes were incubated for 2 h in the presence of 10 μg of GA (G) per ml or in DMSO (D) or left untreated (U) prior to heat shock at 30°C for the times indicated. The HSE-binding activities of HSF1 were then compared by gel mobility shift assay. (B) Gel mobility shift assay of oocytes that were treated with GA or DMSO as described above, heat shocked at 33°C for 1 h and then allowed to recover at 18°C for the periods of time indicated. The positions of activated HSF1 and nonspecific (ns) bands are indicated at the left of each panel.