Figure 3. Bioinformatics analysis of OsGP1.

(A), Nucleotide sequence and deduced amino acid sequence of OsGP1 coding region. (B) Secondary structure prediction. Blue, α-helix (h); green, β-turn (t); red, extended strand (e); purple, random coil (c). (C) Tertiary structure prediction. The model was constructed with Q851X5_ORYSJ as the template. The Global Model Quality Estimate (GMQE) score is 0.62 and the colours represent different model confidence scores. (D) Prediction of conserved domains of OsGP1 protein. Red, signal peptide; blue, transmembrane region; purple, low complexity region. (E) Prediction of the signal peptide. The S-score is higher in the signal peptide region, the C-score is highest at the cleavage site, the Y-score is a parameter that comprehensively considers the S-score and C-score, and the Y-max score is the putative cleavage site. (F) Protein interaction relationship. The red node is OsGP1 (OsJ_13402), and the nodes in other colors represent the ten proteins predicted to interact with OsGP1. Different coloured lines represent different interaction types. Black, co-expression; purple, experimentally determined; yellow, textmining. (G) Analysis of OsGP1 promoter. All cis-acting elements selected for display have a PlantCARE matrix score ≥5.