FIGURE 6.

FTO overexpression relieves OGD/R cell injury by inhibiting mitochondrial functions via the FYN/Drp1 axis. (A) Western blot to measure FYN, p‐Drp1 Ser616, and mitochondrial Drp1 levels; (B) CCK‐8 to examine the cell viability; (C) TUNEL to detect the cell apoptosis; (D) Flow cytometry to detect cell apoptosis; (E) Mito‐Sox staining to test mitochondrial ROS levels; (F) TEM to assess mitochondrial conditions; (G) Mitochondrial length detection; (H) ELISA to detect MDA and SOD expression; (I) ATP detection kits to check ATP levels; (J) Iron content kits to examine Fe²⁺ content; (K) Western blot to measure GPX4 and 4HNE expression. *p < 0.05 versus the OGD/R + oe‐NC + DMSO group, ^# p < 0.05 versus the OGD/R + oe‐FTO + DMSO group. Kruskal–Wallis test was adopted for data comparisons among multiple groups. The experiment was repeated thrice.