FIG. 6.

Coimmunoprecipitation of SF3a66 with anti-Tat-SF1 antibody; Western blot of protein preparations from HeLa nuclear extract probed with anti-SF3a66 MAb. NaCl washes (150 mM) of nuclear extract were incubated with PAS bound with preimmune serum (lane 3), anti-Tat-SF1 antibody (lane 4), or anti-SF3a60 antibody (lane 5). Samples (with Sepharose) were boiled and separated on an SDS–10% polyacrylamide gel. Lane 2 is total nuclear extract (NE) not purified via PAS. Positions of molecular mass standards (m) are indicated in kilodaltons.