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. 2024 Mar 2;73(4):62. doi: 10.1007/s00262-024-03652-2

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Changes in the proportion of immune cells analyzed by flow cytometry. A Representative flow cytometric images showed gating strategy and immune cell clustering. B Bar chart showed the proportion of M1 macrophages in CD45 + cells of each group. (labeled as ***, p < 0.001; ****, p < 0.0001). C Bar chart showed the cell proportion of M2 macrophages in CD45 + cells of each group(labeled as **, p < 0.01; ***, p < 0.001; ****, p < 0.0001). D Bar chart showed M1/M2 ratio in each group(labeled as *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001). E–H Bar chart showed the cell proportion of T cells, CD4 + T cells, CD8 + T cells, and neutrophils in CD45 + cells (labeled as *, p < 0.05; ***, p < 0.001). I, J Bar chart showed the plasma concentration of IL-18 and IFN-γ in each group (*, p < 0.05)