Fig. 2.

Role of riboflavin in driving TAMs morphology and polarization. A Schematic overview of the biochemical link between Riboflavin (vitamin B2) and LSD1 enzyme. B At left, riboflavin levels expressed as peak area in S- and L-TAM populations in the 14 TAM pairs. At right, LSD1 activity of S- and L-TAM pairs obtained from 8 CLM-patients expressed as OD. C LSD1 expression analyses in S- and L-TAMs (n = 4 CLM patients). Gene expressions were normalized using GAPDH as a housekeeping gene. D At left, LSD1 expression levels in M1 and M2 in-vitro polarized monocytes. At right, LSD1 activity of M1 and M2 polarized monocytes expressed as OD. Gene expressions were normalized using GAPDH as a housekeeping gene. Gene expression data are represented as mean ± SD of the average of three biological replicates. *p < 0.05, **p < 0.01 Wilcoxon Mann–Whitney test