Fig. 3.

Gene analysis on patient-derived tumor cells highlighted high TNFα expression. A Representative immunohistochemistry images showing a CLM patient with higher S-TAMs (left) and higher LTAMs (right) in the peritumoral area. Scale bar: 30 µm. B TNFα and TGFβ expression in primary cells from patients with higher S-TAMs (n = 3) and with higher L-TAMs (n = 3). C LSD1 expression in primary cells from patients with higher S-TAMs (n = 3) and with higher L-TAMs (n = 3). Gene expressions were normalized using GAPDH as a housekeeping gene. Data are represented as mean ± SD of the average of three biological replicates (Mann–Whitney test; *p < 0.05)