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. 2024 Mar 2;15:1947. doi: 10.1038/s41467-024-46180-4

Fig. 1. GREB1 expression in the human endometrium is regulated by steroid hormones.

Fig. 1

a Representative images of GREB1 staining in human endometrium from the proliferative (n = 9) and secretory (n = 9) phase. White arrow, GREB1-positive cells; red arrow, GREB1-negative cells. Right panel represents staining with the isotype control Rabbit IgG. b GREB1 raw transcript scores in mid-secretory phase endometrium from women with and without recurrent implantation failure (RIF) measured from a publicly available GEO data set (GSE65102); (n = 10). Paired, two-tailed, t-test. Data reported as the mean ± SEM. *P < 0.05, ***P < 0.001, ****P < 0.0001. Relative amounts of GREB1 and FOXO1 mRNA (c), and GREB1 protein (d) in human endometrial stromal cells treated with 1 µM MPA for the indicated number of hours. Analyzed by one-way ANOVA with Tukey’s multiple comparisons post-test. Data reported as the mean ± SEM. *P < 0.05, ***P < 0.001, ****P < 0.0001. e Representative GREB1 immunofluorescence in human endometrial stromal cells treated with 1 µM MPA for 4 hr. Blue, DAPI; Green, GREB1; and Red, Phalloidin, data are reported as the mean ± SEM from three biological replicates from a representative experiment (experiment repeated three times). *P < 0.05, ***P < 0.001, ****P < 0.0001.