. 2023 Jul 26;76(1):103–111. doi: 10.1007/s12070-023-04092-7

Table 4.

Calculation of percentage of positive cells, staining intensity and staining index

The percentage of p53/CK19 positive cells( A)	The staining intensity (B)	The staining index = Percentage of immunopositive cells(A) multiplied by intensity score (B)i.e. staining index = AX B
• 0% = No positive cells • 1% = 1–33% positive cells • 2% = 34–66% positive cells • 3% = 67–100% positive cells	• 0% = When there was no staining. • 1% = In parts where positivity was observed only at a magnification of 40x. • 2% = In cases where the staining intensity was obvious at 10x, but not at 4x. • 3% = In fields where immunopositive cells were seen even at 4x.	0 = Zero( Absent) 1,2 = Low 3,4 = Moderate/ Medium 6–9 = High

The percentage of p53/CK19 positive cells( A)

The staining intensity (B)

The staining index =
Percentage of immunopositive cells(A) multiplied by intensity score (B)i.e. staining index = AX B

• 0% = No positive cells

• 1% = 1–33% positive cells

• 2% = 34–66% positive cells

• 3% = 67–100% positive cells

• 0% = When there was no staining.

• 1% = In parts where positivity was observed only at a magnification of 40x.

• 2% = In cases where the staining intensity was obvious at 10x, but not at 4x.

• 3% = In fields where immunopositive cells were seen even at 4x.

0 = Zero( Absent)

1,2 = Low

3,4 = Moderate/ Medium

6–9 = High