Table 2.

Results of central composite design of three variables

Run	Yeast extract (%)	Time (h)	MnSO4 (%)	EPS (g/L)
				Actual value	Predicted value
1	5	37	0.009	3.38	3.41
2	5	48	0.009	3.24	3.57
3	3.5	43	0.007	2.39	2.33
4	3.5	22	0.007	1.67	1.10
5	5	15	0.009	2.46	2.37
6	5	37	0.014	2.53	2.55
7	6.5	22	0.012	5.15	4.87
8	3.5	22	0.012	1.63	2.01
9	6.5	43	0.012	4.42	5.08
10	7.5	37	0.009	6.44	6.01
11	5	37	0.009	3.70	3.41
12	5	37	0.005	1.28	1.44
13	6.5	22	0.007	1.49	2.09
14	5	37	0.009	3.44	3.41
15	5	37	0.009	3.40	3.41
16	6.5	43	0.007	4.30	3.96
17	5	37	0.009	3.34	3.41
18	3.5	43	0.012	2.47	1.59
19	2.5	37	0.009	1.39	2.01
20	5	37	0.009	3.32	3.41