Table 3. The amplification procedures of PCR system.
| Temperature | Time | |
|---|---|---|
| Pre-denaturation | 95 °C | 1 min |
| Denaturation | 95 °C | 20 s |
| Annealing | 55 °C | 20 s |
| Extendsion | 72 °C | 30 s |
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