Fig. 3.

Halicin remains active against Staphylococcus aureus-Xen36 biofilms grown on ultra-high molecular weight polyethylene (UHMWPE) discs. a) Less mature 24-hour biofilms and b) more mature seven-day biofilms on UHMWPE discs were exposed to the indicated concentrations of halicin (red symbols) or vancomycin (blue symbols) for 20 hours. Effects on biofilm viability were determined by CFU assays. Dashed lines connect medians of four to seven independent experiments for each drug concentration. Each symbol denotes the median for each drug concentration from an independent experiment, with two to three UHMWPE discs per symbol. MIC, minimum inhibitory concentration.