FIG. 6.

DNA-binding specificity of Tbx2. A series of WT and mutant oligonucleotides based on the MSEu or MSEi was used in band shift assays (C) together with ITT Tbx2. The probes and competitors are indicated. Competitors were used at 10, 50, and 250 ng. The sequences or the probes and competitors are shown in panel A along with the full sequence of the parental MSEu or MSEi oligonucleotide. The derivatives used in the competition assays are identical except for the indicated residues shown in lowercase. ^mG, methylated G residue; ^mC, methylated C residue; I, inosine. For ease of reference, the bases within the GTGTGA motif are numbered 1 to 6. (B) Summary of the binding assays shown in panel C. Only the bound DNA is shown.