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. 2024 Feb 28;35:549–563. doi: 10.1016/j.bioactmat.2024.02.003

Fig. 6.

Fig. 6

ECM stiffness regulates glutamine metabolism of osteogenesis by YAP. (A) Glutamine uptake and (B) GLS activity in MSCs on soft and stiff ECM on days 1 and 7 (n = 4 for each group). (C) The gene expression of SLC1A5 and GLS in MSCs on days 1 and 7 (n = 3 for each group). (D and E) Glutamine uptake (D, n = 4 for 1 d, 6 for 7 d) and GLS activity (E, n = 4 for each group) in MSCs with knockdown of YAP (siYAP) on days 1 and 7. (F and G) The gene expression levels of SLC1A5 (F, n = 5 for each group) and GLS (G, n = 7 for 1 d, 8 for 7 d) in MSCs transfected with siCO or siYAP on days 1 and 7. (H) Left, representative immunofluorescence of YAP in MSCs treated with 20 μM BPTES for 6 h, scale bar, 25 μm. Right, quantification of nuclear YAP localization (n = 30 for each group). (I and J) The gene expression levels of ANKRD1 (I) and CTGF (J) in MSCs cultured with 20 μM BPTES for 6 h (n = 4 for each group). Data from at least three independent experiments. All graphs showed mean ± s.d. Statistical significance was derived from unpaired two-tailed Student's t-test.