Fig. 10.

VP40 and VP40 mutant phenotypes in COS-7 cells. A: Representative images of WT-EGFP-VP40 and EGFP-VP40 mutants 14 h posttransfection in COS-7 cells. Scale bars are 10 μm. B: Percentage of the cell population expressing detectable plasma membrane localization 14 h posttransfection, normalized to WT EGFP-VP40 plasma membrane localization. Three independent experiments were performed with at least 100 cells counted for each experiment. WT VP40 pre-VLPs detectable in cells were normalized to 100% to compare changes in pre-VLP detection for mutant constructs. Average values are shown ± the SEM, ∗ P value < 0.05.