Fig. 8.

Number and Brightness analysis to determine VP40 oligomerization in live cells. A: Representative image frame from the 100 images acquired for each imaging experiment. All images were collected at the same zoom (16.4). Scale bar = 10 μm. B: Normalized VP40 oligomer formation. To compare changes between WT VP40 and mutations, the average EGFP-VP40 population determined for monomer-hexamer, hexamer-12mer, and 12mer+ was normalized to 100% for each condition. WT and mutant EGFP-VP40 oligomer population values are shown ± the SEM, ∗ P value < 0.05.