Fig. 9.

VP40 C-terminal domain lysine residues are important for PI(4,5)P₂ binding. A: Representative SDS PAGE of the LUV pelleting assay to measure VP40 binding to control (1,2-dipalmitoyl-sn-glycero-3-phosphocholine and cholesterol (50:50)) or vesicles containing 5% PI(4,5)P₂. Both vesicle mixtures had a trace amount of dansylPE for visualization of the lipid vesicle pellet. Molecular weight is labeled for each VP40 protein gel panel above and below the VP40 band in kDa. B: Average fraction bound to LUVs shown ± the SEM, ∗ P value < 0.05. Three independent replicates were performed for each protein construct.