FIGURE 3.

Stimulation of WNK‐SPAK‐NKCC1 complex in BCAS mouse brains. (A) Representative immunoblots of increased WNK‐SPAK/OSR1‐NKCC1 complex proteins and decreased myelin basic proteins (MBP) in BCAS‐induced hypoperfused mouse brains (membrane protein fractions) at 4 weeks after surgery. Na‐K pump (α subunit) was used as a loading control. (B–D) Quantitative analyses. Data are mean ± SEM; Student's t test; n = 3–4, *p < 0.05. B, BCAS; BCAS, bilateral carotid artery stenosis; CC, corpus callosum; CTX, cortex; HP, hippocampus; S, sham; STR, striatum.