FIG. 3.

Acetylation status of histone H3. Cross-linked and fragmented chromatin preparations from wild-type (+), rpd3 (R), sin3 (S), and ume6 (U) strains were immunoprecipitated with the antibodies to generally acetylated (Ac) histone H3 or to nonacetylated (UnAc) H3; as a control, the analysis was performed prior to immunoprecipitation (Total). Recovered DNA was analyzed by quantitative PCR as described in the legend to Fig. 2.