TABLE 2.
Mapping the domain of repressed chromatina
| Promoter region | Yeast strain
|
|||
|---|---|---|---|---|
| Wild type | rpd3 | sin3 | ume6 | |
| 750U | 1.0 | 1.0 ± 0.1 | 0.8 ± 0.1 | 1.3 ± 0.1 |
| 600U | 1.0 | 1.3 ± 0.1 | 1.3 ± 0.4 | 1.6 ± 0.8 |
| 450U | 1.0 | 1.6 ± 0.1 | 1.7 ± 0.5 | 3.0 ± 0.5 |
| 250U | 1.0 | 2.1 ± 0.1 | 2.8 ± 0.2 | 3.1 ± 0.2 |
| 100U | 1.0 | 2.4 ± 0.2 | 3.1 ± 0.1 | 3.3 ± 0.4 |
| URS1 | 1.0 | 2.9 ± 0.1 | 2.5 ± 0.2 | 2.3 ± 0.7 |
| 100D | 1.0 | 2.5 ± 0.3 | 2.6 ± 0.3 | 2.9 ± 0.2 |
| 200D | 1.0 | 1.6 ± 0.1 | 1.8 ± 0.1 | 2.0 ± 0.2 |
| 300D | 1.0 | 1.1 ± 0.2 | 0.9 ± 0.1 | 1.8 ± 0.1 |
| 550D | 1.0 | 0.8 ± 0.1 | 0.9 ± 0.2 | 1.1 ± 0.3 |
| 800D | 1.0 | 1.1 ± 0.1 | 0.9 ± 0.2 | 1.2 ± 0.1 |
| 1550D | 1.0 | 1.3 ± 0.1 | 1.2 ± 0.1 | 1.0 ± 0.1 |
a
Each entry represents analysis of cross-linked and fragmented chromatin from the indicated yeast strain that was immunoprecipitated with the antibodies to acetylated lysine 5 of histone H4. Each PCR mixture contained primers for the indicated part of the promoter region (see Fig. 1) and primers for the internal LacZ control; each value represents the ratio (average of two determinations ± error) of the promoter region to the LacZ region for that specific PCR mixture. Values are normalized to that obtained with the wild-type strain, which is defined as 1.0.