FIG. 2.

Expression of SNURF mRNA in rat and human tissues. (A) Northern blot of various rat tissues. Polyadenylated RNA samples (5 μg/lane) from testis (lane 1), prostate (lane 2), seminal vesicles (lane 3), brain (lane 4), spleen (lane 5), and kidney (lane 6). (B) Northern blot of poly(A)⁺ RNA (2 μg/lane) from various human tissues. Human multiple tissue Northern blot II (Clontech) contains RNA from spleen (lane 1), thymus (lane 2), prostate (lane 3), testis (lane 4), ovary (lane 5), small intestine (lane 6), colon (lane 7), and peripheral blood leukocytes (lane 8). Both blots were probed with ³²P-labeled cDNA fragments of SNURF as described in Materials and Methods. To check the integrity of the RNA samples, the rat blot was reprobed with rat γ-actin, and the human blot was reprobed with human β-actin cDNA.